Biochem/physiol Actions

Reversible: no

Primary TargetMobile ion carrier for Ca2+.

Product does not compete with ATP.

Cell permeable: yes

General description

Highly specific for divalent cations (Ca²⁺ > Mg²⁺ >> Sr²⁺ = Ba²⁺). More effective than antibiotic A23187 as a mobile ion carrier for Ca²⁺. Complexes with Ca²⁺ between pH 7 and 9.5, causing significant absorption in the UV range; however, in contrast to A23187 (Cat. No. 100105), it is not fluorescent. Useful for studies of Ca²⁺ transport across biological membranes and measurement of cytoplasmic free Ca²⁺. Induces apoptotic neuronal degeneration in embryonic cortical neurons. Also induces cell cycle arrest in the G₁ interval of mature Burkitt lymphoma cell lines.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Aagaard-Tillery, K.M., and Jelinek, D.F. 1995. J. Immunol.155, 3297.Takei, N., and Endo, Y. 1994. Brain Res. 652, 65.Dupuy d'Angeac, A., et al. 1993. J. Cell Physiol.154, 44.Lee, H.C. 1993. J. Biol. Chem.268, 293.Kuznetsov, G., et al. 1992. J. Biol. Chem. 267, 3932.Toeplitz, B.K., et al. 1979. J. Am. Chem. Soc.101, 3344.Liu, C.M., and Hermann, T.E. 1978. J. Biol. Chem.253, 5892.

Packaging

1 mg in Plastic ampoule

Packaged under inert gas

Physical form

A 5 mM (1 mg/282 µl) solution of Ionomycin, Free Acid, Streptomyces conglobatus (Cat. No. 407950) in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Irritant (B)